中国水稻科学 ›› 2012, Vol. 26 ›› Issue (6): 737-740.DOI: 10.3969/j.issn.10017216.2012.06.014

• 研究简报 • 上一篇    下一篇

利用染色体片段置换系定位水稻赖氨酸含量的QTL

林静 ,朱镇 ,方先文 ,王艳平 ,张所兵 ,王才林*   

  1. 江苏省农业科学院 粮食作物研究所/江苏省种质资源保护与利用研究中心/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心, 江苏 南京 210014;
  • 收稿日期:2011-12-30 修回日期:2012-02-09 出版日期:2012-11-10 发布日期:2012-11-10
  • 通讯作者: 王才林*
  • 基金资助:

    农业部行业科研专项(200803056); 江苏省自主创新项目\[cx(09)634\]; 江苏省自主创新项目(CX(11)1020);江苏省重大科技支撑计划资助项目(BE2008354); 江苏省产学研前瞻性联合研究项目(BY2010134)。

Mapping of QTLs for  Rice Lysine Content Using Chromosome Segment Substitution Lines

LIN Jing, ZHU Zhen, FANG Xianwen, WANG Yanping, ZHANG Suobing, WANG Cailin*   

  1. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Research Center for Protection and Utilization of Agricultural Germplasm Resource in Jiangsu Province/Jiangsu High Quality Rice R&D Center/Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, China;
  • Received:2011-12-30 Revised:2012-02-09 Online:2012-11-10 Published:2012-11-10
  • Contact: WANG Cailin*

摘要: 以籼稻品种9311为受体、粳稻品种日本晴为供体构建95个染色体片段置换系, 对水稻控制赖氨酸含量的QTL进行了定位。结果显示, 共有7个染色体片段置换系(chromosome segment substitution line, CSSL)的稻米赖氨酸含量与亲本9311差异显著。利用代换作图法共鉴定了4个与赖氨酸含量相关的QTL, 分别位于水稻第8、9和12染色体上。其中qHLY8和qHLY9.2来自高赖氨酸含量籼稻品种9311, 正向加性效应百分率分别为9.6%和8.5%;而qHLY9.1和qHLY12则来自低赖氨酸含量粳稻品种日本晴, 负向加性效应百分率分别为-16.0%和-21.3%。

关键词: 染色体片段置换系, 赖氨酸含量, 代换作图, 水稻

Abstract: A total of 95 chromosome segment substitution lines (CSSLs) derived from 9311 and Nipponbare was used to detect quantitative trait loci (QTLs) controlling the lysine content of rice. The result showed that the lysine content of seven CSSLs differed from   that of 9311. Totally four QTLs  for the lysine content of rice were preliminary mapped on the chromosomes 8, 9 and 12 by substitution mapping. Among these QTLs, qHLY8 and qHLY9.2 were both contributed by the indica variety 9311 with high lysine content with the positive effect percentage being 9.6% and 8.5%, respectively; while  qHLY9.1and  qHLY12 were derived from the japonica variety Nipponbare with low lysine content with the negative effect percentage of -160% and -21.3%, respectively.

Key words: chromosome segment substitution lines, lysine content, substitution mapping, rice

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